genistein induces apoptosis and inhibits proliferation of ht29 colon cancer cells
نویسندگان
چکیده
soybean isoflavone genistein has multiple anticancer properties and its pro-apoptotic and anti-proliferative effects have been studied in different cancer cells. however, the mechanisms of action of genistein and its molecular targets on human colon cells have not been fully elucidated. therefore, caspase-3 and p38 mitogen-activated protein kinase (p38 mapk) as the main therapeutic targets were investigated in this study at both gene expression and protein levels in ht29 colon cancer cells. the caspase-3 and p38 mapk gene expression levels were examined by real time pcr whereas flow cytometry technique was performed to determine their intracellular protein levels. the caspase-3 enzyme activity was obtained by colorimetric method while the gelatinase activity of matrix metalloproteinase-2 (mmp2) was determined by zymography. in addition, mtt test, wound healing assay and clonogenic assay were carried out to determine the effect of genistein on ht29 cell viability, migration, and proliferation, respectively. genistein induced apoptotic death in ht29 cells through activation of caspase-3 pathway at the transcriptional, protein, and enzymatic levels. moreover, genistein inhibited the proliferation of ht29 cells by reducing of both p38 mapk gene expression and its active phosphorylated protein level. also, we showed that genistein strongly suppressed the metastatic potency of ht29 colon cancer cells via the reduction of mmp2 activity. based on the results of this study, we conclude that genistein may exhibit its anticancer properties on ht29 colon cancer cells by modulating caspase-3 and p38 mapk pathway at different transcriptional and protein levels.
منابع مشابه
Genistein Induces Apoptosis and Inhibits Proliferation of HT29 Colon Cancer Cells
Soybean isoflavone genistein has multiple anticancer properties and its pro-apoptotic and anti-proliferative effects have been studied in different cancer cells. However, the mechanisms of action of genistein and its molecular targets on human colon cells have not been fully elucidated. Therefore, caspase-3 and p38 mitogen-activated protein kinase (p38 MAPK) as the main therapeutic targets...
متن کاملGenistein Induces Apoptosis and Inhibits Proliferation of HT29 Colon Cancer Cells
Soybean isoflavone genistein has multiple anticancer properties and its pro-apoptotic and anti-proliferative effects have been studied in different cancer cells. However, the mechanisms of action of genistein and its molecular targets on human colon cells have not been fully elucidated. Therefore, caspase-3 and p38 mitogen-activated protein kinase (p38 MAPK) as the main therapeutic targets were...
متن کاملDownregulation of Kinesin Spindle Protein Inhibits Proliferation, Induces Apoptosis and Increases Chemosensitivity in Hepatocellular Carcinoma Cells
Background: Kinesin spindle protein (KSP) plays a critical role in mitosis. Inhibition of KSP function leads to cell cycle arrest at mitosis and ultimately to cell death. The aim of this study was to suppress KSP expression by specific small-interfering RNA (siRNA) in Hep3B cells and evaluate its anti-tumor activity. Methods: Three siRNA targeting KSP (KSP-siRNA #1-3) and one mismatched-siRNA (...
متن کاملFermented wheat aleurone inhibits growth and induces apoptosis in human HT29 colon adenocarcinoma cells.
Fermentation of dietary fibre by the gut microflora may enhance levels of SCFA, which are potentially chemoprotective against colon cancer. Functional food containing wheat aleurone may prevent cancer by influencing cell cycle and cell death. We investigated effects of fermented wheat aleurone on growth and apoptosis of HT29 cells. Wheat aleurone, flour and bran were digested and fermented in v...
متن کاملAnalgesic-antitumor peptide induces apoptosis and inhibits the proliferation of SW480 human colon cancer cells
Colorectal cancer is one of the most common malignant tumors, and is associated with significant morbidity and mortality. In this study, recombinant analgesic-antitumor peptide (rAGAP), a protein consisting of small ubiquitin-related modifier (SUMO) linked with a hexa-histidine tag, was used as an antitumor analgesic peptide. The purpose of the present study was to investigate the antitumor act...
متن کاملResveratrol Treatment Inhibits Proliferation of and Induces Apoptosis in Human Colon Cancer Cells
BACKGROUND Resveratrol, a natural isolate from plant sources, has a long and important history in traditional Chinese medicine. In the present study we investigated the effect of resveratrol on human colon cancer cell lines. MATERIAL/METHODS We used the Cell Counting kit-8 (CCK-8) for determination of colon cancer cell viability. Apoptosis induction was analyzed using the DeadEnd™ Colorimetri...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
international journal of molecular and cellular medicineجلد ۵، شماره ۳، صفحات ۱۷۸-۱۹۱
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023